US scientists have partially revived pig brains four hours after the animals were slaughtered.
The findings could fuel debate about the barrier between life and death, and provide a new way of researching diseases like Alzheimer’s.
The study showed the death of brain cells could be halted and that some connections in the brain were restored.
However, there were no signals from the brain that would indicate awareness or consciousness.
The surprise findings challenge the idea that the brain goes into irreversible decline within minutes of the blood supply being cut off.
How was it done?
Thirty-two pig brains were collected from an abattoir.
Four hours later the organs were connected to a system made by the team at Yale University.
It rhythmically pumped (to mimic the pulse) a specially designed liquid round the brain, which contained a synthetic blood to carry oxygen and drugs to slow or reverse the death of brain cells.
The pig brains were given the restorative cocktail for six hours.
What did the study show?
The study, published in the journal Nature, showed a reduction in brain cell death, the restoration of blood vessels and some brain activity.
The researchers found working synapses – the connections between brain cells that allow them to communicate.
The brains also showed a normal response to medication and used up the same amount of oxygen as a normal brain.
This was all 10 hours after the pigs were decapitated.
Crucially there was no sign of the brain-wide electrical activity in an electroencephalogram (EEG brain scan) that would signal awareness or perception.
Fundamentally they were still dead brains.
Are these experiments ethical?
The pig brains came from the pork industry; the animals were not raised in a lab for this experiment.
But the Yale scientists were so concerned the pigs might become conscious that they gave drugs to the disembodied brains to reduce any brain activity.
And the team were constantly monitoring the brains to see if there was any sign of higher brain functions.
In that case they would have used anaesthetic and ended the experiment.
Ethicists, writing in Nature, say new guidelines are needed for this field because animals used for research could end up in a “grey area – not alive, but not completely dead”.
What is the point?
The immediate benefit of this work will be for scientists studying the brain in diseases like Alzheimer’s.
The organ is the most complex structure in the known universe, but techniques such as freezing slices of the brain or growing colonies of brain cells in a dish do not let researchers explore the full 3D wiring of the brain.
In the long term, scientists hope to find better ways of protecting the brain after traumas such as a stroke or being starved of oxygen at birth.
Dr Andrea Beckel-Mitchener, from the Brain Initiative at the US National Institute of Mental Health, said: “This line of research could lead to a whole new way of studying the post-mortem brain.
“It also could stimulate research to develop interventions that promote brain recovery after loss of brain blood flow.”
However, the researchers say it is still far too early for the field to make a difference to patients after injury.
Prof Sestan said: “We don’t yet have knowledge whether we would be able to restore normal brain function.”